Super粪便基因组DNA快速提取试剂盒

1423-Super粪便基因组DNA快速提取试剂盒

目录号：1423

目录编号	包装单位
142301	50次

 
适用范围：
适用于快速提取各种粪便DNA

产品介绍：
常规的DNA纯化方式并不能有效地去除粪便中存在的大量抑制因子而导致下游实验的失败，如PCR不能扩增出所需片段。该试剂盒采用DNA吸附柱和新型独特的溶液系统，能有效去除动物粪便中各种影响下游实验（如PCR）的抑制因子，并能高效地回收粪便中的基因组DNA。动物粪便样品经特殊缓冲液ASL重悬后，70℃处理5分钟裂解细菌；离心去除不溶解的杂质，蛋白酶K消化进一步去除蛋白和杂质；然后基因组DNA在高离序盐状态下选择性吸附于离心柱内硅基质膜， 再通过一系列快速的漂洗－离心的步骤， 抑制物去除液和漂洗液将细胞代谢物、蛋白等杂质去除， 最后低盐的洗脱缓冲液将纯净基因组DNA从硅基质膜上洗脱。
 
产品特点：
1.离心吸附柱内硅基质膜全部采用进口世界著名公司特制吸附膜，柱与柱之间吸附量差异极小，可重复性好。克服了国产试剂盒膜质量不稳定的弊端。
2.不需要使用有毒的苯酚等试剂，也不需要乙醇沉淀等步骤。
3.快速，简捷，单个样品操作一般可在40分钟内完成。
4.多次柱漂洗确保高纯度，OD₂₆₀/OD₂₈₀典型的比值达1.7～1.9，可直接用于PCR，Southern-blot和各种酶切反应。

The DNA Stool Mini Kit provides fast and easy purification of total DNA from fresh or frozen stool samples. Purified DNA is of high quality and well suited for use in PCR and other downstream enzymatic reactions. The simple spin procedure yields pure DNA ready for direct use in less than 1 hour. Purification requires no phenol–chloroform extraction or alcohol precipitation, and involves minimal handling. DNA is eluted in low-salt buffer and is free of protein, nucleases, and other impurities or inhibitors. The purified DNA is ready for use in PCR and other enzymatic reactions, or can be stored at –20°C for later use. 
     Stool samples typically contain many compounds that can degrade DNA and inhibit downstream enzymatic reactions. To ensure removal of these substances, the DNA Stool Mini Kit contains Buffer AB which efficiently adsorb these substances early in the purification process so that they can easily be removed by a quick centrifugation step. In addition, the kit contains Buffer ASL, which is specially developed to remove inhibitory substances from stool samples. 
      DNA of up to 20 kb is purified by the QIAamp DNA Stool Mini Kit. DNA of this length denatures completely during thermal cycling and can be amplified with the highest efficiency.