无内毒素质粒小提试剂盒
EndoFree Plasmid Mini Kit
目录号：1204
|
目录编号 |
包装单位 |
|
120401 |
20次 |
|
120402 |
50次
|
产品介绍：
本试剂盒采用改进SDS-碱裂解法裂解细胞，通过独特的内毒素清除剂选择性结合离心除去内毒素，然后离心吸附柱内的硅基质膜在高盐、低pH值状态下选择性地结合溶液中的质粒DNA，再通过去蛋白液和漂洗液将杂质和其它细菌成分去除，最后低盐、高pH值的洗脱缓冲液将纯净质粒DNA从硅基质膜上洗脱。
产品特点：
1.离心吸附柱内硅基质膜全部采用进口世界著名公司特制吸附膜，柱与柱之间吸附量差异极小，可重复性好。克服了国产试剂盒膜质量不稳定的弊端。
2.独特工艺配方清除内毒素，内毒素含量极低（<10 EU/μg DNA），细胞转染效果极佳。也可直接用于酶切、转化、PCR、体外转录、测序、等各种分子生物学实验。
The EndoFree Miniprep system provides a fast, simple, and cost-effective plasmid miniprep method for EndoFree plasmid. Miniprep Kits use a modified alkaline lysis method and silica membrane technology. Endotoxins and other impurities are removed by simple temperature-mediated extraction and phase separation. Plasmid DNA purified with EndoFree Miniprep Kits is immediately ready for use. The plasmid DNA is suitable for a variety of experiments in molecular biology, including transfection, automated fluorescent DNA sequencing, restriction enzyme digestion, or transformation.
