用于PAXgene全血RNA保存管提取血液RNA
目录号：1149
产品介绍：
PAX Blood RNA Kit用于配套 PAXgene Blood RNA Tubes使用，PAXgene Blood RNA Tubes采集血样后可以在18–25°C条件下可稳定多至3天，在–20°C或–70°C条件下至少稳定60个月。本产品可以提取保存在采血管的全血中分离和纯化胞间RNA，与采样、稳定及纯化形成整合的系统。PAX Blood RNA系统包括用于血液采集、稳定和运输的PAXgene Blood RNA Tubes（由BD提供，货号762165），以及基于硅胶膜技术、离心柱法分离纯化的PAX Blood RNA Kit。纯化可使用离心机进行手工操作，也可在QIAcube全自动核酸纯化仪上自动运行。该产品表现卓越，确保高度可靠的RNA纯化。
适用范围：
适用于配套 PAXgene Blood RNA Tubes使用，提取保存在采血管的全血中分离和纯化胞间RNA。
PAX Blood RNA Kit is designed for isolation of total RNA from blood samples stored in preservation reagents and PAXgene® Blood RNA Tubes. This procedure completely removes contaminants and enzyme inhibitors producing high-quality RNA. RNA purified using the PAX Blood RNA Kit is ready for applications such as RT-PCR.
The samples are removed from the preservation reagents. For blood samples stored in PAXgene® Blood RNA Tubes, the cells are collected by centrifugation. Samples are washed and lysed under an optimized buffer containing Proteinase K. The samples are centrifuged to remove cell debris and other particulates. After adjusting the binding conditions with ethanol, the samples are loaded on the RNA binding column. With a brief centrifugation or vacuum step, the samples pass through the column matrix which binds the RNA. Genomic DNA is removed with an on-the-column DNase I digestion treatment. After three wash steps, purified RNA is eluted with RNase-free water.
