病毒RNA快速提取试剂盒
目录号：1127
适用范围：
适用于快速提取病毒RNA
产品介绍：
采用特异性结合病毒RNA 的离心吸附柱和独特的缓冲液系统，病毒RNA提取试剂盒适合于从无细胞体液，包括血浆、血清、腹水、培养细胞上清液、脑脊髓液及尿液等中快速提取高纯的病毒RNA。该产品可以满足绝大多数的病毒RNA的提取要求， 如病毒RNA：HCV（丙肝病毒）,HIV（艾滋病毒）,和HTLV（人类嗜T淋巴细胞病毒）；等等。病毒裂解后，RNA后在高离序盐状态下选择性吸附于离心柱内硅基质膜，再通过一系列快速的漂洗－离心的步骤，将盐、细胞代谢物、蛋白等杂质去除，最后低盐的洗脱缓冲液将纯净的病毒RNA从硅基质膜上洗脱。纯化后的病毒核酸无杂质和PCR抑制剂，可直接适用于PCR/RT-PCR等分析。
产品特点：
1.不需要使用有毒的苯酚等试剂，也不需要乙醇沉淀等步骤。
2.节省时间，简捷，单个样品操作一般可在20分钟内完成。
3.多次柱漂洗确保高纯度，提取的病毒RNA 纯度高，质量稳定可靠，可适用于各种常规操作，包括PCR/RT-PCR等。
Viral RNA Mini Kits provide the fastest and easiest way to purify viral RNA for reliable use in amplification technologies. Viral RNA can be purified from plasma (treated with anticoagulants other than heparin), serum, and other cell-free body fluids. Samples may be fresh or frozen, but if frozen, should not be thawed more than once. Repeated freeze–thawing of plasma samples will lead to reduced viral titers and should be avoided for optimal sensitivity. Cryoprecipitates accumulate when samples are subjected to repeated freeze–thaw cycles. This may lead to clogging of the membrane when using the vacuum protocol. Viral RNA Mini Kits are for general use and can be used for isolation of viral RNA from a wide variety of viruses, but performance can not be guaranteed for every virus.
Viral RNA Mini Kits represent a well established technology for general-use viral RNA preparation. The kit combines the selective binding properties of a silica based membrane with the speed of microspin or vacuum technology and is highly suited for simultaneous processing of multiple samples. The sample is first lysed under highly denaturing conditions to inactivate RNases and to ensure isolation of intact viral RNA. Buffering conditions are then adjusted to provide optimum binding of the RNA to the silica membrane, and the sample is loaded onto the Mini spin column. The RNA binds to the membrane, and contaminants are efficiently washed away in two steps using two different wash buffers. High-quality RNA is eluted in a RNase-free water. The purified RNA is free of protein, nucleases, and other contaminants and inhibitors.
