RNApure超纯总RNA快速提取试剂盒

1103- RNApure超纯总RNA快速提取试剂盒

  目录号：1103

目录编号	包装单位
110301	20次
110302	50次

适用范围：
适用于快速提取各种动植物细胞/组织高纯度总RNA

产品介绍：
改进的异硫氰酸胍/酚一步法裂解细胞和灭活RNA 酶， 然后总RNA在高离序盐状态下选择性吸附于离心柱内硅基质膜， 再通过一系列快速的漂洗－离心的步骤, 去蛋白液和漂洗液将细胞代谢物、蛋白等杂质去除， 最后低盐的RNase free water将纯净RNA从硅基质膜上洗脱。

产品特点：
1.离心吸附柱内硅基质膜全部采用进口世界著名公司特制吸附膜，柱与柱之间吸附量差异极小，可重复性好。克服了国产试剂盒膜质量不稳定的弊端。
2.结合了异硫氰酸胍/酚一步法试剂稳定性好，纯度高和离心柱方便快捷的优点，不需要异丙醇沉淀和乙醇洗涤过程，RNA可以直接从离心柱上洗脱避免了过度干燥不易溶解问题。
3.独有的RL裂解液配方，可以有效的消除基因组污染。
4.多次漂洗去蛋白过程，提取RNA纯度更高。
5.有效的去除了5S在总RNA中含量，提高了纯度。

The RNApure Total RNA Kit Kit provides a simple, reliable, and rapid method for isolating high-quality total RNA from a variety of samples, including animal and plant cells and tissue . The kit utilizes the strong lysis capability of Buffer RL(TRIzol® Reagent), followed by a convenient and time-saving silica-column purification protocol to purify ultrapure total RNA within an hour. 
    The sample is first lysed with Buffer RL, according to the lysate preparation protocol provided. The addition of chloroform to your sample, followed by centrifugation separates the solution into an upper aqueous phase containing RNA and a lower phenol-containing organic phase. The upper aqueous phase is transferred to a new tube, followed by ethanol addition and centrifugation. The sample is then transferred to the Spin Column containing a clear silica–based membrane to which the RNA binds during purification. The RNA is washed to remove contaminants and the purified total RNA is then eluted in RNase-Free Water (Tris Buffer, pH 7.5 may also be used) and is suitable for use in a variety of downstream applications including sensitive gene expression studies such as microarray analysis or real time quantitative RT-PCR (qRT-PCR).